Composite

Part:BBa_K5311019:Design

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_026 + RNA thermometer ForU + mCherry + His-tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 612
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 612
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by using the corresponding sequences on this registry: MG26:BBa_K5311000; Part: RNA Thermometer: Part:BBa_K115002; mCherry: Part:BBa_K5311008 and the His-tag corresponds to a sequence of 6 Hystidines


Source

For this composite part we have codon optimized the CDS for the mCherry on the RNA Thermometer to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly.

References